|
|
|
|
|
| Lédo,Ana da Silva; Santana,Fernanda Vieira; Oliveira,Annie Carolina Araújo de; Oliveira,Leila Albuquerque Resende de; Silva,Ana Veruska Cruz da. |
| ABSTRACT: This study evaluated the effect of vitrification solutions and exposure time on the cryopreservation of Brazilian green dwarf coconut plumules (BGD) using the droplet vitrification technique. Explants were excised from BGD mature fruits from the Active Germplasm Bank of Embrapa Tabuleiros Costeiros, Sergipe, Brazil. Firstly, embryos were disinfected, and after excision, plumules were pre-cultivated for 72 hours in Y3 + 0.6 M sucrose + 2.2 g L-1 Gelrite® culture medium. Plumules were exposed to PVS2 and PVS3 solutions for 15 and 30 minutes and rapidly immersed in liquid nitrogen (-196 ºC). After cryopreservation, they were thawed in culture medium solution (Y3 + 1.2 M sucrose) and cultured in regeneration medium. The experimental design was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cocos nucifera L.; Cryoprotection; PVS2; PVS3. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000100401 |
| |
|
| |
|
|
| Cardoso,Milena Nascimento; Araújo,Aparecida Gomes de; Oliveira,Leila Albuquerque Resende; Cardoso,Bruno Trindade; Muniz,Ana Veruska Cruz da Silva; Santos,Paulo Sérgio Neves dos; Oliveira,Annie Carolina Araújo de; Machado,Caroline de Araújo; Ledo,Ana da Silva. |
| ABSTRACT: The objective of this research was to evaluate the proline synthesis and physiological response of cassava genotypes which were micro propagated and induced to salinity stress in vitro. Micro cuttings of approximately 1.0cm long with a single bud of genotypes TBRS Tapioqueira, BRS Verdinha and Lagoão which were previously established in vitro were inoculated in a MS medium containing different concentrations of NaCl (0; 25; 50; 75; 100mM) and were analyzed after 90th day for: number of roots, number of leaves and shoot dry mass. The proline content of BRS Tapioqueira and Lagoão was assessed at 30th, 60th and 90th day. There was no analysis of proline of the variety Verdinha because of the contamination of the explants. The experimental design... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta; Abiotic stress; Osmoprotectors. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600401 |
| |
|
|
|
